45點自動對焦系統可用的自動對焦點數量、類型及分布因使用鏡頭而有所不同，共分為A至H八組，可從下表查看所使用的鏡頭所屬組別。使用E至H組鏡頭時的可用自動對焦點較少。

	對應f/2.8 雙十字型自動對焦點
	對應f/5.6 十字型自動對焦點
	對應f/5.6 垂直自動對焦點 (對水平線條敏感)
	對應f/5.6 水平自動對焦點 (對垂線條敏感)
	無法使用的自動對焦點

A 組

45個十字型自動對焦點
(包括1個雙十字型自動對焦點)

EF 14mm f/2.8L II USM
EF 14mm f/2.8L USM
EF 15mm f/2.8 Fisheye
EF 20mm f/2.8 USM
EF 24mm f/1.4L II USM
EF 24mm f/1.4L USM
EF 24mm f/2.8 IS USM
EF 24mm f/2.8
EF 28mm f/1.8 USM
EF 28mm f/2.8 IS USM
EF 28mm f/2.8
EF 35mm f/1.4L II USM
EF 35mm f/1.4L USM
EF 35mm f/2 IS USM
EF 35mm f/2
EF 40mm f/2.8 STM
EF 50mm f/1.0L USM
EF 50mm f/1.2L USM
EF 50mm f/1.4 USM
EF 50mm f/1.8 STM

EF 50mm f/1.8 II
EF 50mm f/1.8
EF 85mm f/1.2L II USM
EF 85mm f/1.2L USM
EF 85mm f/1.4L IS USM
EF 85mm f/1.8 USM
EF 100mm f/2 USM
EF 135mm f/2L USM
EF 135mm f/2L USM + Extender EF 1.4x I/II/III
EF 135mm f/2.8 (Softfocus)
EF 200mm f/1.8L USM
EF 200mm f/1.8L USM + Extender EF 1.4x I/II/III^#
EF 200mm f/2L IS USM
EF 200mm f/2L IS USM + Extender EF 1.4x I/II/III
EF 200mm f/2.8L II USM
EF 200mm f/2.8L USM
EF 300mm f/2.8L IS II USM
EF 300mm f/2.8L IS USM
EF 300mm f/2.8L USM
EF 400mm f/2.8L IS II USM

EF 400mm f/2.8L IS USM
EF 400mm f/2.8L II USM
EF 400mm f/2.8L USM
TS-E 45mm f/2.8*
TS-E 90mm f/2.8*
EF 16-35mm f/2.8L III USM
EF 16-35mm f/2.8L II USM
EF 16-35mm f/2.8L USM
EF 17-35mm f/2.8L USM
EF 20-35mm f/2.8L
EF 24-70mm f/2.8L II USM
EF 24-70mm f/2.8L USM
EF 28-70mm f/2.8L USM
EF 70-200mm f/2.8L IS III USM
EF 70-200mm f/2.8L IS II USM
EF 70-200mm f/2.8L IS USM
EF 70-200mm f/2.8L USM
EF 80-200mm f/2.8L
EF-S 24mm f/2.8 STM
EF-S 17-55mm f/2.8 IS USM

B 組

45個十字型自動對焦點

EF 50mm f/2.5 Compact Macro
EF 50mm f/2.5 Compact Macro + LIFE SIZE Converter
EF 100mm f/2.8L Macro IS USM
EF 100mm f/2.8 Macro
EF 135mm f/2L USM + Extender EF 2x I/II/III
EF 180mm f/3.5L Macro USM
EF 200mm f/1.8L USM + Extender EF 2x I/II/III^#
EF 200mm f/2L IS USM + Extender EF 2x I/II/III
EF 200mm f/2.8L II USM + Extender EF 1.4x I/II/III
EF 200mm f/2.8L II USM + Extender EF 2x I/II/III
EF 200mm f/2.8L USM + Extender EF 1.4x I/II/III
EF 200mm f/2.8L USM + Extender EF 2x I/II/III
EF 300mm f/2.8L IS II USM + Extender EF 1.4x I/II/III
EF 300mm f/2.8L IS II USM + Extender EF 2x I/II/III
EF 300mm f/2.8L IS USM + Extender EF 1.4x I/II/III
EF 300mm f/2.8L IS USM + Extender EF 2x I/II/III
EF 300mm f/2.8L USM + Extender EF 1.4x I/II/III^#
EF 300mm f/2.8L USM + Extender EF 2x I/II/III^#
EF 300mm f/4L IS USM
EF 300mm f/4L IS USM + Extender EF 1.4x I/II/III
EF 300mm f/4L USM
EF 300mm f/4L USM + Extender EF 1.4x I/II/III
EF 400mm f/2.8L IS II USM + Extender EF 1.4x I/II/III
EF 400mm f/2.8L IS II USM + Extender EF 2x I/II/III
EF 400mm f/2.8L IS USM + Extender EF 1.4x I/II/III
EF 400mm f/2.8L IS USM + Extender EF 2x I/II/III
EF 400mm f/2.8L II USM + Extender EF 1.4x I/II/III^#
EF 400mm f/2.8L II USM + Extender EF 2x I/II/III^#
EF 400mm f/2.8L USM + Extender EF 1.4x I/II/III^#
EF 400mm f/2.8L USM + Extender EF 2x I/II/III^#
EF 400mm f/4 DO IS II USM
EF 400mm f/4 DO IS II USM + Extender EF 1.4x I/II/III
EF 400mm f/4 DO IS USM
EF 400mm f/4 DO IS USM + Extender EF 1.4x I/II/III
EF 400mm f/5.6L USM
EF 500mm f/4L IS II USM
EF 500mm f/4L IS II USM + Extender EF 1.4x I/II/III
EF 500mm f/4L IS USM
EF 500mm f/4L IS USM + Extender EF 1.4x I/II/III
EF 500mm f/4.5L USM

EF 500mm f/4.5L USM
EF 600mm f/4L IS II USM
EF 600mm f/4L IS II USM + Extender EF 1.4x I/II/III
EF 600mm f/4L IS USM
EF 600mm f/4L IS USM + Extender EF 1.4x I/II/III
EF 600mm f/4L USM
EF 600mm f/4L USM + Extender EF 1.4x I/II/III^#
TS-E 17mm f/4L*
TS-E 24mm f/3.5L*
TS-E 24mm f/3.5L II*
EF 8-15mm f/4L Fisheye USM
EF 16-35mm f/4L IS USM
EF 17-40mm f/4L USM
EF 24-70mm f/4L IS USM
EF 24-105mm f/3.5-5.6 IS STM
EF 24-105mm f/4L IS II USM
EF 24-105mm f/4L IS USM
EF 28-80mm f/2.8-4L USM
EF 28-90mm f/4-5.6 III
EF 28-90mm f/4-5.6 II USM
EF 28-90mm f/4-5.6 II
EF 28-90mm f/4-5.6 USM
EF 28-90mm f/4-5.6
EF 28-105mm f/3.5-4.5 II USM
EF 28-105mm f/3.5-4.5 USM
EF 28-135mm f/3.5-5.6 IS USM
EF 28-200mm f/3.5-5.6 USM
EF 28-200mm f/3.5-5.6
EF 28-300mm f/3.5-5.6L IS USM
EF 35-105mm f/3.5-4.5
EF 35-135mm f/3.5-4.5
EF 50-200mm f/3.5-4.5L
EF 50-200mm f/3.5-4.5
EF 70-200mm f/2.8L IS III USM + Extender EF 1.4x I/II/III
EF 70-200mm f/2.8L IS III USM + Extender EF 1.4x I/II/III EF 70-200mm f/2.8L IS III USM + Extender EF 2x I/II/III
EF 70-200mm f/2.8L IS II USM + Extender EF 1.4x I/II/III
EF 70-200mm f/2.8L IS II USM + Extender EF 2x I/II/III
EF 70-200mm f/2.8L IS USM + Extender EF 1.4x I/II/III
EF 70-200mm f/2.8L IS USM + Extender EF 2x I/II/III
EF 70-200mm f/2.8L USM + Extender EF 1.4x I/II/III^
EF 70-200mm f/2.8L USM + Extender EF 2x I/II/III^

EF 70-200mm f/4L IS II USM
EF 70-200mm f/4L IS USM
EF 70-200mm f/4L IS USM + Extender EF 1.4x I/II/III
EF 70-200mm f/4L USM
EF 70-200mm f/4L USM + Extender EF 1.4x I/II/III
EF 70-210mm f/3.5-4.5 USM
EF 70-210mm f/4
EF 70-300mm f/4-5.6L IS USM
EF 70-300mm f/4-5.6 IS II USM
EF 70-300mm f/4-5.6 IS USM
EF 70-300mm f/4.5-5.6 DO IS USM
EF 75-300mm f/4-5.6 IS USM
EF 75-300mm f/4-5.6 III USM
EF 75-300mm f/4-5.6 III
EF 75-300mm f/4-5.6 II USM
EF 75-300mm f/4-5.6 II
EF 75-300mm f/4-5.6
EF 100-200mm f/4.5A
EF 100-300mm f/5.6L
EF 100-300mm f/5.6
EF 100-400mm f/4.5-5.6L IS II USM
EF 100-400mm f/4.5-5.6L IS USM
EF 200-400mm f/4L IS USM Extender 1.4x
EF 200-400mm f/4L IS USM Extender 1.4x + Extender EF 1.4x I/II/III
EF 200-400mm f/4L IS USM Extender 1.4x: 內置增距鏡設定為1.4x
EF-S 35mm f/2.8 Macro IS STM
EF-S 60mm f/2.8 Macro USM
EF-S 10-22mm f/3.5-4.5 USM
EF-S 15-85mm f/3.5-5.6 IS USM
EF-S 17-85mm f/4-5.6 IS USM
EF-S 18-55mm f/3.5-5.6 IS STM
EF-S 18-55mm f/3.5-5.6 IS II
EF-S 18-55mm f/3.5-5.6 III
EF-S 18-135mm f/3.5-5.6 IS USM
EF-S 18-135mm f/3.5-5.6 IS STM
EF-S 18-135mm f/3.5-5.6 IS
EF-S 18-200mm f/3.5-5.6 IS
EF-S 55-250mm f/4-5.6 IS STM
EF-S 55-250mm f/4-5.6 IS II
EF-S 55-250mm f/4-5.6 IS

C 組

35個十字型自動對焦點
10個垂直自動對焦點

TS-E 50mm f/2.8L Macro
TS-E 90mm f/2.8L Macro
TS-E 135mm f/4L Macro
EF 11-24mm f/4L USM
EF 20-35mm f/3.5-4.5 USM

EF 35-135mm f/4-5.6 USM
EF 75-300mm f/4-5.6 USM
EF 100-300mm f/4.5-5.6 USM
EF-S 18-55mm f/3.5-5.6 IS
EF-S 18-55mm f/3.5-5.6 II USM

EF-S 18-55mm f/3.5-5.6 II
EF-S 18-55mm f/3.5-5.6 USM
EF-S 18-55mm f/3.5-5.6

D 組

15個十字型自動對焦點
30個垂直自動對焦點

EF 24-85mm f/3.5-4.5 USM
EF 35-350mm f/3.5-5.6L USM
EF 55-200mm f/4.5-5.6 II USM

EF 55-200mm f/4.5-5.6 USM
EF 80-200mm f/4.5-5.6
EF 90-300mm f/4.5-5.6 USM

EF 90-300mm f/4.5-5.6
EF-S 10-18mm f/4.5-5.6 IS STM
EF-S 18-55mm f/4-5.6 IS STM

E 組

15個十字自動對焦點
20個垂直自動對焦點

EF 100mm f/2.8 Macro USM
EF 800mm f/5.6L IS USM
EF 1200mm f/5.6L USM
EF 28-70mm f/3.5-4.5 II
EF 28-70mm f/3.5-4.5
EF 28-80mm f/3.5-5.6 V USM
EF 28-80mm f/3.5-5.6 IV USM

EF 28-80mm f/3.5-5.6 III USM
EF 28-80mm f/3.5-5.6 II USM
EF 28-80mm f/3.5-5.6 II
EF 28-80mm f/3.5-5.6 USM
EF 28-80mm f/3.5-5.6
EF 35-70mm f/3.5-4.5A
EF 35-70mm f/3.5-4.5

EF 35-80mm f/4-5.6 II
EF 35-80mm f/4-5.6 PZ
EF 38-76mm f/4.5-5.6
EF 80-200mm f/4.5-5.6 II
EF 80-200mm f/4.5-5.6 USM

F 組

9個十字自動對焦點
20個垂直自動對焦點
6個水平自動對焦點

EF 180mm f/3.5L Macro USM + Extender EF 1.4x I/II/III
EF 22-55mm f/4-5.6 USM
EF 28-105mm f/4-5.6 USM

EF 28-105mm f/4-5.6
EF 35-80mm f/4-5.6 III
EF 35-80mm f/4-5.6 USM

EF 35-80mm f/4-5.6

G 組(f/8)

9個十字自動對焦點
18個垂直自動對焦點

EF 100-400mm f/4.5-5.6L IS II USM + Extender EF 1.4x III

EF 200-400mm f/4L IS USM Extender 1.4x + Extender EF 2x III

H 組

1個十字自動對焦點

EF 35-105mm f/4.5-5.6 USM

EF 35-105mm f/4.5-5.6

H 組(f/8)

1個十字自動對焦點

EF 300mm f/4L IS USM + Extender EF 2x I/II/III
EF 300mm f/4L USM + Extender EF 2x I/II/III
EF 400mm f/4 DO IS II USM + Extender EF 2x I/II/III
EF 400mm f/4 DO IS USM + Extender EF 2x I/II/III
EF 400mm f/5.6L USM + + Extender EF 1.4x I/II/III
EF 500mm f/4L IS II USM + Extender EF 2x I/II/III
EF 500mm f/4L IS USM + Extender EF 2x I/II/III

EF 500mm f/4.5L USM + Extender EF 1.4x I/II/III^#
EF 600mm f/4L IS II USM + Extender EF 2x I/II/III
EF 600mm f/4L IS USM + Extender EF 2x I/II/III
EF 600mm f/4L USM + Extender EF 2x I/II/III^#
EF 800mm f/5.6L IS USM + Extender EF 1.4x I/II/III
EF 1200mm f/5.6L USM + Extender EF 1.4x I/II/III^#
EF 70-200mm f/4L IS USM + Extender EF 2x I/II/III

EF 70-200mm f/4L USM + Extender EF 2x I/II/III
EF 100-400mm f/4.5-5.6L IS II USM + Extender EF 1.4x I/II
EF 100-400mm f/4.5-5.6L IS USM + Extender EF 1.4x I/II/III
EF 200-400mm f/4L IS USM Extender 1.4x + Extender EF 2x I/II
EF 200-400mm f/4L IS USM Extender 1.4x: 內置增距鏡設定為1.4x + Extender EF 1.4x I/II/III

f/8 十字自動對焦點

注意：EF 180mm f/3.5L Macro USM鏡頭安裝Extender EF2x (I/II/III)時，不能進行自動對焦。
# 與Extender EF 1.4x III 或 EF 2x III配合使用時，可能無法以自動對焦實現精確對焦。
^ 此鏡頭及增距鏡組合可能無法以自動對焦實現精確對焦。
* 當使用TS-E鏡頭手動對焦至合焦時，觀景器中的對焦指示燈會亮起並發出嗶聲。自動對焦組別只適用於TS-E鏡頭無傾斜/偏移的情況。